| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mummert, Lauren E (14)
|
MACYA |
2:25.11 |
2:21.64 |
-3.47 |
| 2 |
Sopko, Micah N (13)
|
MACYA |
2:30.72 |
2:27.77 |
-2.95 |
| 3 |
Yeager, Rebecca E (13)
|
MAHAC |
2:30.64 |
2:27.86 |
-2.78 |
| 4 |
Fackler, Katie (14)
|
MAHAC |
2:29.79 |
2:29.15 |
-0.64 |
| 5 |
Miller, Gabrielle M (13)
|
MACYA |
2:30.71 |
2:29.33 |
-1.38 |
| 6 |
Mummert, Katherine N (14)
|
MACYA |
2:29.07 |
2:31.82 |
2.75 |
| 7 |
Eichman, Olivia A (13)
|
MAHAC |
2:39.21 |
2:38.43 |
-0.78 |
| 8 |
Uhlin, Brina (13)
|
MADSC |
|
2:40.23 |
- |
| 9 |
Carrasco, Katharine M (14)
|
MADSC |
2:41.22 |
2:41.07 |
-0.15 |
| 10 |
Kratz, Breanna F (14)
|
MAHAC |
2:54.49 |
2:50.23 |
-4.26 |
| 11 |
Hollenbach, Bella P (13)
|
MAMARA |
3:06.25 |
3:02.26 |
-3.99 |